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Three-Dimensional Electron Microscopy of Macromolecular AssembliesVisualization of Biological Molecules in Their Native State$
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Joachim Frank

Print publication date: 2006

Print ISBN-13: 9780195182187

Published to Oxford Scholarship Online: April 2010

DOI: 10.1093/acprof:oso/9780195182187.001.0001

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Interpretation of Three-Dimensional Images of Macromolecules

Interpretation of Three-Dimensional Images of Macromolecules

(p.277) 6 Interpretation of Three-Dimensional Images of Macromolecules
Three-Dimensional Electron Microscopy of Macromolecular Assemblies

Joachim Frank

Oxford University Press

This chapter covers all aspects of interpretation, starting with validation and consistency checks. Methods for visualization of the reconstruction by surface rendering are discussed, along with criteria for the definition of molecular boundaries. Segmentation is described both based on intrinsic properties of the reconstructed density distribution and on the use of tags such as gold labels or antibodies in the experiment. The chapter goes on to describe how a quasi-atomic model may be built by the fitting and docking of atomic coordinates into the 3D density map.

Keywords:   consistency checks, docking, fitting, molecular boundaries, quasi-atomic model, segmentation, surface rendering, visualization

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