Validating Flow-Through Respirometry
Validating Flow-Through Respirometry
This chapter describes methods for validating the measurements made using flow-through respirometry. These methods include the injection of nitrogen at a known flow rate into a respirometry system; burning a small flame of ethanol or methanol at a rate measured by weighing the lamp (often called “alcohol recovery”); and burning hydrocarbon gases such as methane, ethane, or propane. The first two techniques yield information on absolute oxygen consumption rates; the gas flame technique yields information on ratios between oxygen consumption and carbon dioxide production, as does the alcohol recovery technique. Full practical details including all relevant equations are given.
Keywords: metabolic measurement validation, alcohol recovery, calibration, validation, gas flow technique
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