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Measuring Metabolic RatesA Manual for Scientists$
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John R. B. Lighton

Print publication date: 2008

Print ISBN-13: 9780195310610

Published to Oxford Scholarship Online: September 2008

DOI: 10.1093/acprof:oso/9780195310610.001.0001

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Validating Flow-Through Respirometry

Validating Flow-Through Respirometry

(p.132) 12 Validating Flow-Through Respirometry
Measuring Metabolic Rates

John R. B. Lighton

Oxford University Press

This chapter describes methods for validating the measurements made using flow-through respirometry. These methods include the injection of nitrogen at a known flow rate into a respirometry system; burning a small flame of ethanol or methanol at a rate measured by weighing the lamp (often called “alcohol recovery”); and burning hydrocarbon gases such as methane, ethane, or propane. The first two techniques yield information on absolute oxygen consumption rates; the gas flame technique yields information on ratios between oxygen consumption and carbon dioxide production, as does the alcohol recovery technique. Full practical details including all relevant equations are given.

Keywords:   metabolic measurement validation, alcohol recovery, calibration, validation, gas flow technique

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