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Measuring Metabolic RatesA Manual for Scientists$
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John R. B. Lighton

Print publication date: 2008

Print ISBN-13: 9780195310610

Published to Oxford Scholarship Online: September 2008

DOI: 10.1093/acprof:oso/9780195310610.001.0001

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Constant Volume and Constant Pressure Respirometry

Constant Volume and Constant Pressure Respirometry

(p.7) 2 Constant Volume and Constant Pressure Respirometry
Measuring Metabolic Rates

John R. B. Lighton

Oxford University Press

This chapter describes constant pressure (Gilson) and constant volume (Warburg) respirometry — long-established techniques that are still capable of accurate results. These are useful for measuring the metabolic rates of small organisms, cell cultures, and biochemical preparations. In Gilson respirometry, oxygen consumption is measured by reducing the volume of a sealed system to maintain a constant pressure. In Warburg respirometry, oxygen consumption is measured by quantifying the accompanying drop in the pressure of a sealed system, the volume of which is known. The theory and practical details of implementing both techniques are described in detail. Finally, a more modern, computerized alternative to Warburg respirometry is introduced that can be implemented at low cost.

Keywords:   Gilson, Warburg, metabolic measurement, oxygen consumption

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