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Measuring Metabolic RatesA Manual for Scientists$
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John R. B. Lighton

Print publication date: 2008

Print ISBN-13: 9780195310610

Published to Oxford Scholarship Online: September 2008

DOI: 10.1093/acprof:oso/9780195310610.001.0001

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Constant Volume Techniques Using Gas Analysis

Constant Volume Techniques Using Gas Analysis

(p.23) 4 Constant Volume Techniques Using Gas Analysis
Measuring Metabolic Rates

John R. B. Lighton

Oxford University Press

By using modern gas analyzers and variations of constant volume techniques described in Chapter 2, simple and high-throughput measurement of the metabolic rates of organisms ranging in size from bacteria to large insects and even small vertebrates are easily implemented. It is even possible to measure water loss rate and carbon dioxide production using only an oxygen analyzer. The techniques can be deployed in the field as well as the laboratory. Both manual and automated, computerized implementations of constant volume techniques are covered in full step-by-step detail, and appropriate analytical protocols for oxygen, carbon dioxide, or both oxygen and carbon dioxide analysis systems are also described in detail.

Keywords:   oxygen consumption, respirometry, carbon dioxide, gas analyzers

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