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Measuring Metabolic RatesA Manual for Scientists$
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John R. B. Lighton

Print publication date: 2008

Print ISBN-13: 9780195310610

Published to Oxford Scholarship Online: September 2008

DOI: 10.1093/acprof:oso/9780195310610.001.0001

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Aquatic Oxygen Analysis

Aquatic Oxygen Analysis

(p.47) 5 Aquatic Oxygen Analysis
Measuring Metabolic Rates

John R. B. Lighton

Oxford University Press

Measuring oxygen consumption rates in aquatic media is the only practical method for determining the metabolic rates of cell cultures and aquatic organisms. This chapter describes the two principal variations of aquatic respirometry — closed and open system (or flow-through) respirometry — together with procedures for calibrating aquatic respirometry systems, acquiring data from them, and analyzing the resulting data. The chapter also describes the operation of the widely used Clark dissolved oxygen electrode, the characteristics of common electrode membrane materials, and necessary routine maintenance. There is coverage of common problems, and trouble-shooting guides are included.

Keywords:   dissolved oxygen, aquatic respirometry, oxygen electrode, Clark electrode

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