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Measuring Metabolic RatesA Manual for Scientists$
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John R. B. Lighton

Print publication date: 2008

Print ISBN-13: 9780195310610

Published to Oxford Scholarship Online: September 2008

DOI: 10.1093/acprof:oso/9780195310610.001.0001

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Flow-Through Respirometry: The Equations

Flow-Through Respirometry: The Equations

(p.100) 9 Flow-Through Respirometry: The Equations
Measuring Metabolic Rates

John R. B. Lighton

Oxford University Press

This chapter de-mystifies respirometry equations, showing how they can be derived using a simple mental trick: concentrating the analysis on the principal gas that is neither consumed nor produced by animals. The effect of dilution of oxygen by carbon dioxide, the enrichment of carbon dioxide by the consumption of oxygen, and the effects of water vapor on the concentrations of both gases, are described and quantified. A system of eight equations is derived that allow oxygen consumption and carbon dioxide production to be calculated in practically any feasible flow-through respirometry system.

Keywords:   respirometry equations, oxygen consumption, carbon dioxide production, dilution, water vapor

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