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Neurovascular MedicinePursuing Cellular Longevity for Healthy Aging$
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Kenneth Maiese

Print publication date: 2009

Print ISBN-13: 9780195326697

Published to Oxford Scholarship Online: January 2010

DOI: 10.1093/acprof:oso/9780195326697.001.0001

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PRINTED FROM OXFORD SCHOLARSHIP ONLINE (oxford.universitypressscholarship.com). (c) Copyright Oxford University Press, 2020. All Rights Reserved. An individual user may print out a PDF of a single chapter of a monograph in OSO for personal use. date: 03 December 2020

Glutamatergic Signaling In Neurogenesis

Glutamatergic Signaling In Neurogenesis

(p.269) Chapter 11 Glutamatergic Signaling In Neurogenesis
Neurovascular Medicine

Noritaka Nakamichi

Yukio Yoneda

Oxford University Press

This chapter summarizes recent studies on the functional expression of ionotropic glutamate receptors (iGluRs) and metabotropic glutamate receptors (mGluRs) by neural progenitor cells isolated from embryonic rat and mouse brains. Cells are cultured in the presence of growth factors toward the formation of round spheres termed as neurospheres for different periods under floating conditions, whereas a reverse transcription polymerase chain reaction (RT-PCR) analysis reveals expression of messenger RNA for particular iGluR and mGluR subtypes in undifferentiated cells and neurospheres formed with clustered cells during the culture with growth factors. Sustained exposure to an agonist for the N-methyl-D-aspartate (NMDA) receptor not only inhibits the formation of neurospheres but also promotes spontaneous and induced differentiation of neurospheres into cells immunoreactive to a neuronal marker protein on immunocytochemistry and Western blot analyses. This chapter shows that glutamate could play a pivotal role in mechanisms underlying proliferation for self-renewal.

Keywords:   neural progenitor cells, neurospheres, NMDA receptors, metabotropic glutamate receptors, neurogenesis, ionotropic glutamate receptors, growth factors, glutamate, self-renewal

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