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From Molecules to Living Organisms: An Interplay Between Biology and PhysicsLecture Notes of the Les Houches School of Physics: Volume 102, July 2014$
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Eva Pebay-Peyroula, Hugues Nury, François Parcy, Rob W. H. Ruigrok, Christine Ziegler, and Leticia F. Cugliandolo

Print publication date: 2016

Print ISBN-13: 9780198752950

Published to Oxford Scholarship Online: March 2016

DOI: 10.1093/acprof:oso/9780198752950.001.0001

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PRINTED FROM OXFORD SCHOLARSHIP ONLINE (oxford.universitypressscholarship.com). (c) Copyright Oxford University Press, 2022. All Rights Reserved. An individual user may print out a PDF of a single chapter of a monograph in OSO for personal use.date: 28 June 2022

NMR spectroscopy: from basic concepts to advanced methods

NMR spectroscopy: from basic concepts to advanced methods

(p.134) 5 NMR spectroscopy: from basic concepts to advanced methods
From Molecules to Living Organisms: An Interplay Between Biology and Physics

Enrico Luchinat

Oxford University Press

This chapter aims to give a general overview of the field of NMR spectroscopy applied to structural biology. In the first section the basic concepts of NMR theory are outlined, together with some technical aspects, from nuclear spin to multidimensional experiments. In the second section more advanced applications of protein NMR are presented, including methods for studying large proteins, fast-pulsing techniques, carbon-detected NMR, paramagnetic NMR and solid-state NMR. The final section focuses on recent advances in NMR applied to structural biology of proteins: in-cell NMR. With this method, high-resolution NMR is used to investigate proteins at atomic detail in living cells. The main effects of the intracellular environment on protein structure and dynamics are explained. Several in-cell NMR approaches are discussed: protein expression in bacterial cells, protein insertion or expression into higher eukaryotes. Some applications of protein expression by transient transfection in human cells are given.

Keywords:   NMR spectroscopy, in-cell NMR, NMR theory, nuclear spin, structural biology, protein expression, atomic detail, living cells

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