This chapter introduces optoelectronics tweezers (OET) and its applications in manipulating micro- and nano-scale particles in fluidic environment. It starts with a brief overview of various particle manipulation techniques, including optical tweezers, dielectrophoresis and OET. It is followed by a detailed discussion of OET operation principle, device structures, theoretical models, optimal liquid conductivity, and experimental results on the manipulation of a variety of micro and nanoparticles such as bacteria, mammalian cells, and nanowires. Numerous OET enabled microfluidic functions such as massively parallel single particle manipulation, microparticle sorting using dynamic optical images, real-time interactive control of OET, and micro/nanoparticle patterning are also included. Recent advance in single crystalline OET that is capable of manipulating biological cells in high conductivity physiological media is also discussed.
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