Ancestral Sequence Reconstruction
David A Liberles
Abstract
Ancestral sequence reconstruction is a technique of growing importance in molecular evolutionary biology and comparative genomics. As a powerful tool for testing evolutionary and ecological hypotheses, as well as uncovering the link between sequence and molecular phenotype, there are potential applications in almost all fields of applied molecular biology. This book starts with a historical overview of the field, before discussing the potential applications in drug discovery and the pharmaceutical industry. This is followed by a section on computational methodology, which provides a detailed d ... More
Ancestral sequence reconstruction is a technique of growing importance in molecular evolutionary biology and comparative genomics. As a powerful tool for testing evolutionary and ecological hypotheses, as well as uncovering the link between sequence and molecular phenotype, there are potential applications in almost all fields of applied molecular biology. This book starts with a historical overview of the field, before discussing the potential applications in drug discovery and the pharmaceutical industry. This is followed by a section on computational methodology, which provides a detailed discussion of the available methods for reconstructing ancestral sequences (including their advantages, disadvantages, and potential pitfalls). Purely computational applications of the technique are then covered, including whole proteome reconstruction. Further chapters provide a detailed discussion on taking computationally reconstructed sequences and synthesizing them in the laboratory. The book concludes with a description of the scientific questions where experimental ancestral sequence reconstruction has been utilized to provide insights and inform future research.
Keywords:
sequence-function mapping,
substitution,
proteome reconstruction,
genome,
evolution,
phylogenetics,
bioinformatics,
drug discovery,
maximum likelihood,
parsimony
Bibliographic Information
| Print publication date: 2007 |
Print ISBN-13: 9780199299188 |
| Published to Oxford Scholarship Online: September 2008 |
DOI:10.1093/acprof:oso/9780199299188.001.0001 |
Authors
Affiliations are at time of print publication.
David A Liberles, editor
Department of Molecular Biology, University of Wyoming, USA
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