Crystals, X-rays and Proteins: Comprehensive Protein Crystallography
Dennis Sherwood and Jon Cooper
Abstract
This book presents a complete account of the theory of the diffraction of X-rays by crystals with particular reference to the processes of determining the structures of protein molecules. The book develops from first principles all relevant mathematics, diffraction, and wave theory. The practical aspects of sample preparation and X-ray data collection using both laboratory and synchrotron sources are covered along with data analysis at both the theoretical and practical levels. The important role played by the Patterson function in structure analysis by both molecular replacement and experimen ... More
This book presents a complete account of the theory of the diffraction of X-rays by crystals with particular reference to the processes of determining the structures of protein molecules. The book develops from first principles all relevant mathematics, diffraction, and wave theory. The practical aspects of sample preparation and X-ray data collection using both laboratory and synchrotron sources are covered along with data analysis at both the theoretical and practical levels. The important role played by the Patterson function in structure analysis by both molecular replacement and experimental phasing approaches is covered, as are methods for improving the resulting electron density map. The theoretical basis of methods used in refinement of protein crystal structures are then covered in depth along with the crucial task of defining the binding sites of ligands and drug molecules. The complementary roles of other diffraction methods which reveal further detail of great functional importance in a crystal structure are outlined.
Keywords:
X-ray crystallography,
proteins,
diffraction,
symmetry,
Fourier transform,
electron density,
solving the phase problem,
refinement
Bibliographic Information
| Print publication date: 2010 |
Print ISBN-13: 9780199559046 |
| Published to Oxford Scholarship Online: January 2011 |
DOI:10.1093/acprof:oso/9780199559046.001.0001 |
Authors
Affiliations are at time of print publication.
Dennis Sherwood, author
Managing Director, The Silver Bullet Machine Manufacturing Company
Jon Cooper, author
Department of Medicine, University College, London
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