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Crystals, X-rays and ProteinsComprehensive Protein Crystallography$
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Dennis Sherwood and Jon Cooper

Print publication date: 2010

Print ISBN-13: 9780199559046

Published to Oxford Scholarship Online: January 2011

DOI: 10.1093/acprof:oso/9780199559046.001.0001

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Experimental techniques: sample preparation

Experimental techniques: sample preparation

(p.343) 10 Experimental techniques: sample preparation
Crystals, X-rays and Proteins

Dennis Sherwood

Jon Cooper

Oxford University Press

This chapter discusses the basic molecular biology of cloning a gene of interest and expressing the corresponding protein in a suitable host. It covers the principles underlying protein purification by ion exchange, gel filtration and other methods with common methods of crystal screening, and the underlying principles of crystallisation are described. It outlines the main procedures for mounting crystals for X-ray data collection.

Keywords:   protein expression, purification, chromatography, assay, crystallisation, hanging drop method, vapour diffusion method, seeding, crystal mounting

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