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Silent WitnessForensic DNA Evidence in Criminal Investigations and Humanitarian Disasters$
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Henry Erlich, Eric Stover, and Thomas J. White

Print publication date: 2020

Print ISBN-13: 9780190909444

Published to Oxford Scholarship Online: November 2020

DOI: 10.1093/oso/9780190909444.001.0001

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PRINTED FROM OXFORD SCHOLARSHIP ONLINE (oxford.universitypressscholarship.com). (c) Copyright Oxford University Press, 2022. All Rights Reserved. An individual user may print out a PDF of a single chapter of a monograph in OSO for personal use.date: 29 January 2022

Recent Developments in Forensic DNA Technology

Recent Developments in Forensic DNA Technology

Chapter:
(p.105) Chapter 5 Recent Developments in Forensic DNA Technology
Source:
Silent Witness
Author(s):

Henry Erlich

Cassandra Calloway

Steven B. Lee

Publisher:
Oxford University Press
DOI:10.1093/oso/9780190909444.003.0006

The current standard of forensic DNA analysis is genotyping the length polymorphism of STR loci by capillary electrophoresis and analyzing the polymorphism of mitochondrial DNA by Sanger sequencing. However, the trend of dramatic technological developments begun in the mid 1980s has continued, with the most consequential recent innovations being (1) the development of next generation sequencing (NGS) or massively parallel sequencing (MPS) and (2) the implementation of commercial Rapid DNA instruments that automate genotyping of all CODIS core STR loci from sample to profile in 90 minutes. This chapter reviews the principles, benefits, and applications of NGS or MPS technology, with a focus on the critical features of massively parallel and clonal sequencing and the ability to perform quantitative analysis of mixtures. The potential to analyze degraded DNA by using NGS/MPS to sequence mitochondrial DNA and SNPs is discussed, as are the benefits and limitations of Rapid DNA instruments.

Keywords:   next generation sequencing, massively parallel sequencing, NGS, MPS, Rapid DNA, mitochondrial DNA, SNP, STR, degraded DNA, trace DNA

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