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Single-Molecule Studies of Nucleic Acids and Their Proteins - Oxford Scholarship Online
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Single-Molecule Studies of Nucleic Acids and Their Proteins

David Bensimon, Vincent Croquette, Jean-François Allemand, Xavier Michalet, and Terence Strick


This book presents a comprehensive overview of the foundations of single-molecule studies, based on manipulation of the molecules and observation of these with fluorescent probes. It first discusses the forces present at the single-molecule scale, the methods to manipulate them, and their pros and cons. It goes on to present an introduction to single-molecule fluorescent studies based on a quantum description of absorption and emission of radiation due to Einstein. Various considerations in the study of single molecules are introduced (including signal to noise, non-radiative decay, triplet st ... More

Keywords: single-molecule manipulation methods, fluorescence spectroscopy, elasticity and dynamics of polymers, structural transitions in DNA, DNA/RNA polymerase, DNA helicase, topoisomerase, single-molecule DNA sequencing

Bibliographic Information

Print publication date: 2018 Print ISBN-13: 9780198530923
Published to Oxford Scholarship Online: February 2019 DOI:10.1093/oso/9780198530923.001.0001


Affiliations are at time of print publication.

David Bensimon, author
Research Director and Professor, LPS-ENS, PSL, CNRS and Department of Chemistry and Biochemistry, UCLA, USA

Vincent Croquette, author
Research Director, LPS-ENS, PSL, CNRS, France

Jean-François Allemand, author
Professor, LPS-ENS, PSL, CNRS, France